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Gentamicin as a bactericidal antibiotic in tissue culture
Medical Microbiology and Immunology
|January 1, 1975
Summary
Gentamicin affects mammalian cell physiology, impacting proliferation and metabolism at higher doses. It demonstrates superior bactericidal efficiency in tissue cultures compared to penicillin-streptomycin, with minimal impact on cell karyotype.
Area of Science:
- Cell Biology
- Microbiology
- Pharmacology
Background:
- Gentamicin is an antibiotic commonly used in cell culture.
- Its effects on mammalian cell physiology and bactericidal efficiency require thorough investigation.
Purpose of the Study:
- To evaluate the impact of gentamicin on mammalian cell lines.
- To compare its bactericidal efficacy against common bacterial contaminants in tissue culture.
Main Methods:
- Assessed cytotoxicity, proliferation, and metabolic changes (lactate production, LDH release, pH) in 9 mammalian cell lines.
- Examined karyotype stability after prolonged gentamicin exposure.
- Determined minimal bactericidal concentrations (MBC) in cell-free media and tissue cultures against various bacteria.
Main Results:
- Gentamicin induced cytotoxicity and metabolic alterations at concentrations above 125 µg/ml, with sensitivity varying based on cell growth phase.
- No significant karyotype changes were observed.
- Gentamicin exhibited superior bactericidal activity compared to penicillin-streptomycin in tissue cultures.
Conclusions:
- Gentamicin influences mammalian cell physiology, necessitating careful dose selection.
- It is an effective agent for controlling bacterial contamination in tissue culture systems.