Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interpretation of DNA profiles using a computerised database.

P Gill1, D J Werrett

  • 1Central Research and Support Establishment, Home Office Forensic Science Service, Reading, Berkshire, England.

Electrophoresis
|May 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improved deconvolution of sequenced based mixtures by extending EuroForMix.

Forensic science international. Genetics·2026
Same author

Preimplantation genetic testing for aneuploidy mosaicism reporting lacks clinical predictive value for live birth in a multisite, double-blinded study with independent validation.

American journal of obstetrics and gynecology·2025
Same author

Effectiveness of duodenal bulb biopsies in histological diagnosis of coeliac disease.

European journal of pediatrics·2025
Same author

Uptake and experience of professional interpreting services in primary care in a South Asian population: a national cross-sectional study.

BMC primary care·2024
Same author

A qualitative exploration of Maltese couples' care experiences of prostate cancer diagnosis and radiotherapy treatment.

Radiography (London, England : 1995)·2024
Same author

'Low' LRs obtained from DNA mixtures: On calibration and discrimination performance of probabilistic genotyping software.

Forensic science international. Genetics·2024
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

This study introduces an image analysis system for DNA fingerprinting data from autoradiography. The system helps determine band matches in hypervariable loci, improving genetic analysis accuracy.

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Autoradiography is crucial for visualizing DNA fragments in genetic analysis.
  • Hypervariable loci are key markers for individual identification.
  • Accurate interpretation of electrophoretic bands is essential for reliable DNA profiling.

Purpose of the Study:

  • To present an image analysis system for storing and analyzing autoradiography data.
  • To establish a method for objectively determining band matches in DNA fingerprinting.
  • To discuss practical challenges and suggest improvements for DNA analysis systems.

Main Methods:

  • Utilized an image analyzer to store autoradiography data from multi-locus and single-locus hypervariable regions.
  • Examined the electrophoretic system to assign standard deviations to DNA bands.

Related Experiment Videos

  • Developed criteria for determining band matches based on statistical analysis.

    • Main Results:

    • The image analysis system enables data storage and retrieval from autoradiography.
    • Standard deviations can be assigned to bands, facilitating objective band matching.
    • Identified practical limitations in the current system for DNA profiling.

    Conclusions:

    • The developed image analysis system offers a quantitative approach to DNA fingerprinting.
    • Assigning standard deviations to bands improves the reliability of matching.
    • Internal markers are recommended for future system enhancements to increase accuracy.