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Controlled microfluidic switching in arbitrary time-sequences with low drag.

Cassandra S Niman1, Jason P Beech, Jonas O Tegenfeldt

  • 1Nanometer Structure Consortium (nmC@LU) and Division of Solid State Physics, Lund University, Lund, Sweden. cassandra.niman@ftf.lth.se

Lab on a Chip
|May 10, 2013
PubMed
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This summary is machine-generated.

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This study introduces a novel device for precisely controlling biochemical environments around cells and proteins. It enables controlled reagent switching for fundamental cell physiology and molecular interaction studies.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Microfluidics

Background:

  • Studying cell physiology and molecular interactions requires testing responses to changing biochemical environments.
  • Current methods often involve surface-attached cells/molecules with fluid composition changes.
  • Maintaining low flow velocity is crucial for cell/molecule attachment and function.

Purpose of the Study:

  • To develop a device capable of switching multiple biochemical reagents in arbitrary orders.
  • To ensure low flow velocities for cell/molecule stability and function.
  • To characterize fluid interface formation and concentration changes during reagent switching.

Main Methods:

  • Development of a novel device utilizing U-shaped access channels.
  • Application of total-internal reflection fluorescence microscopy for surface concentration analysis.

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  • Utilizing finite-element modeling for device optimization.

    • Main Results:

    • Demonstrated ability to switch biochemical reagents with well-defined fluid interfaces near the surface.
    • Experimental data showed good agreement with Taylor-Aris theory.
    • Achieved distinct switching events and precise control over the biochemical microenvironment.

    Conclusions:

    • The developed device offers precise control over biochemical microenvironments for cell and protein studies.
    • The device facilitates arbitrary reagent switching while maintaining cell/molecule integrity.
    • Key findings are generalizable to various biochemical studies requiring controlled solution switching.