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Updated: May 11, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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dbWFA: a web-based database for functional annotation of Triticum aestivum transcripts.

Jonathan Vincent1, Zhanwu Dai, Catherine Ravel

  • 1INRA, UMR1095 Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, Clermont-Ferrand, F-63 039 Cedex 2, France.

Database : the Journal of Biological Databases and Curation
|May 11, 2013
PubMed
Summary

A new database, dbWFA, streamlines functional gene annotation for wheat (Triticum aestivum) by integrating diverse data sources. This tool accelerates the understanding of wheat gene functions, aiding agricultural research.

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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Area of Science:

  • Plant genomics
  • Bioinformatics
  • Functional gene annotation

Background:

  • Gene annotation in wheat (Triticum aestivum) is challenging due to fragmented data across multiple databases.
  • Mining sequence homology for functional annotation is time-consuming and inefficient.

Purpose of the Study:

  • To develop a dedicated functional annotation database for Triticum aestivum (L.), named dbWFA.
  • To provide a unified and accessible platform for retrieving functional information for wheat genes.

Main Methods:

  • Integrated NCBI UniGene and TriFLDB full-length coding sequences for T. aestivum.
  • Utilized BLAST-based homology searches to link wheat sequences with annotations from model species (Arabidopsis thaliana, Oryza sativa).
  • Incorporated data from Gene Ontology, MapMan, MIPS, PlantCyc, and TAIR.

Main Results:

  • Successfully assigned putative functions to 45% of UniGenes and 81% of TriFLDB full-length coding sequences.
  • Validated annotation accuracy by comparing T. aestivum UniGene annotations with curated model species data.
  • Identified and annotated genes involved in wheat grain development (cell division, storage polymer accumulation).

Conclusions:

  • dbWFA offers an efficient tool for comprehensive functional gene annotation in wheat.
  • The database facilitates prompt information retrieval from multiple annotation systems.
  • Annotation results are consistent with previous wheat transcriptome and proteome studies, confirming dbWFA's utility.