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Updated: May 11, 2026

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published on: July 18, 2011
Mitochondrial degradation during starvation is selective and temporally distinct from bulk autophagy in yeast
Akinori Eiyama1, Noriko Kondo-Okamoto, Koji Okamoto
1Laboratory of Mitochondrial Dynamics, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Abstract:
Selective degradation of mitochondria is a fundamental process that depends on formation of autophagy-related double-membrane vesicles exclusive to mitochondria, and is thus termed mitophagy. In yeast, mitophagy is induced by a shift from respiration to starvation, or prolonged respiratory growth. Here we show that mitochondrial degradation in yeast also occurs selectively under starvation conditions even without respiration. Induction of mitophagy takes place much later than that of bulk autophagy, requiring Atg11 and Atg32 essential for mitophagy as well as Atg17, Atg29, and Atg31 specific for bulk autophagy. We propose that these two discrete protein complexes cooperatively activate starvation-induced mitophagy.
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