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Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

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Related Experiment Video

Updated: May 11, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Engineering T cell function using chimeric antigen receptors identified using a DNA library approach.

Connie P M Duong1, Jennifer A Westwood, Carmen S M Yong

  • 1Cancer Immunology Research Program, Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria, Australia.

Plos One
|May 14, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a new screening method to rapidly identify effective chimeric antigen receptors (CARs) for cancer immunotherapy. This approach identified novel CARs that enhance T cell cancer-killing abilities and inhibit tumor growth in vivo.

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A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
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In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
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Published on: February 27, 2019

Related Experiment Videos

Last Updated: May 11, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
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Published on: May 9, 2025

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
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In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
08:04

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

Area of Science:

  • Immunology
  • Molecular Biology
  • Biotechnology

Background:

  • Genetic engineering of cellular functions offers therapeutic potential for diseases like cancer.
  • Chimeric antigen receptors (CARs) enhance T cell-mediated cancer immunotherapy but require optimization.
  • Current methods for CAR development are slow, relying on testing individual molecular components.

Purpose of the Study:

  • To develop an innovative screening method for rapidly evaluating numerous CAR formats.
  • To identify novel CARs with enhanced anti-cancer activity against tumor-associated antigens.

Main Methods:

  • Constructed a large, directional in-frame library of randomly assembled signaling domains.
  • Developed a CAR specific for the tumor-associated antigen erbB2 using this library.
  • Screened thousands of CAR formats to identify those enhancing T cell anti-cancer functions.

Main Results:

  • Identified several novel and original CAR formats through high-throughput screening.
  • Discovered a specific CAR demonstrating significantly enhanced cancer cell lysis.
  • Demonstrated in vivo efficacy, showing tumor growth inhibition in mouse models.

Conclusions:

  • The developed screening technology accelerates the discovery of effective CARs.
  • This platform can be broadly applied to engineer diverse cellular functions.
  • Novel CARs identified show promise for improving T cell-based cancer therapies.