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Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Published on: May 26, 2023
Vibration-induced pulmonary disease: transformation of the bronchial mucosal epithelium and endothelium
G I Nepomnyashchikh1, O A Postnikova, M A Bakarev
1Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. pathol@soramn.ru
Abstract:
Long-term vibration exposure of humans leads to development of manifest atrophy and structural mosaicism of the bronchial epithelium in the mucosa of the large bronchi (diffuse degeneration of multilamellar cylindrical epithelium, focal hyperplasia, and squamous cell metaplasia). The mucosal subepithelial stroma is characterized by sclerosis, hypoelastosis, and reduction of microvessels. Radioautography confirms the reduction of protein-producing activity of bronchial epitheliocytes and endotheliocytes.
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