[Effect of immobilization on biosensor for benzene derivates detection]
Kuo Tang1, An-Zhou Ma, Qing Yu
1Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. tk054@163.com
Huan Jing Ke Xue= Huanjing Kexue
|May 15, 2013
Summary
Immobilizing Pseudomonas fluorescens A506 whole cell sensors in sodium alginate enhances fluorescent signal speed and peak values. Optimization of cell density and immobilization time significantly improves biosensor performance for benzene detection.
Area of Science:
- Environmental microbiology
- Biosensor technology
Background:
- Whole cell biosensors offer sensitive detection of environmental pollutants.
- Pseudomonas fluorescens A506 (pTS) is a viable whole cell sensor candidate.
- Immobilization techniques can enhance biosensor stability and performance.
Purpose of the Study:
- To optimize the immobilization of Pseudomonas fluorescens A506 (pTS) using sodium alginate.
- To compare the performance of immobilized cells against free cells for benzene detection.
- To identify key factors influencing the immobilized biosensor's signal response.
Main Methods:
- Optimization of cell density, immobilization time, and bead usage for Pseudomonas fluorescens A506 (pTS).
- Immobilization of cells within sodium alginate beads.
- Comparative analysis of fluorescent signal generation between immobilized and free cells.
- Evaluation of biosensor response to varying benzene concentrations.
Main Results:
- Immobilization for 2 hours significantly increased fluorescent signal speed (2.26x) and peak value (2.23x) compared to free cells.
- Lowered cell density and prolonged immobilization time were identified as dominant factors affecting signal intensity.
- Enhanced signal intensity per cell was observed in immobilized cells due to reduced growth.
- The immobilized biosensor demonstrated a more rapid signal response at higher benzene concentrations.
Conclusions:
- Sodium alginate immobilization effectively enhances the performance of Pseudomonas fluorescens A506 (pTS) whole cell biosensors.
- Optimized cell density and immobilization time are crucial for maximizing biosensor sensitivity and response time.
- The immobilized biosensor shows promise for rapid and sensitive detection of benzene in environmental samples.
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