Related Experiment Video
Updated: May 11, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Published on: June 12, 2016
Study of the peak variance in isocratic and gradient liquid chromatography using the transport model
Weiqiang Hao1, Bin Di, Qiang Chen
1High-Tech Research Institute of Nanjing University, Changzhou 213164, China. haowq@hotmail.com
Abstract:
The expressions accounting for the peak variance in isocratic and gradient liquid chromatography is derived from the transport model. In mathematical treatments, the dwelling time is taken into account, and the type of solvent strength, gradient profile and the variation of plate height (H) with mobile phase composition (φ) is not specified. By applying a coordinate transformation, the transport model is solved by using the Laplace transform approach. A plate height equation that is suited for both isocratic and gradient elution is obtained. Based on this equation, the plate height equations for any combination of stepwise and linear gradients are derived. These equations will be algebraic when the solvent strength is linear and the H-φ plot is parabolic. The plate height equations for single stepwise, single linear and the ladder-like gradients are also given.
More Related Videos
07:34Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Related Concept Videos
High-Performance Liquid Chromatography: Elution Process
Column Efficiency: Rate Theory
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...
Chromatographic Methods: Terminology
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
Diffusion on Chromatography Columns
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...