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Published on: February 16, 2020
MR detection of LPS-induced neutrophil activation using mannan-coated superparamagnetic iron oxide nanoparticles
Han Shanhua1, Han Huijing, Myeong Ju Moon
1Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 501-757, South Korea.
Purpose:
The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).
Procedures:
Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.
Results:
The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils in vitro.
Conclusions:
The in vitro labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.
Insights
Mannan-coated superparamagnetic iron oxide nanoparticles (M-SPION) effectively labeled lipopolysaccharide (LPS)-induced neutrophils, showing increased iron uptake. This technique shows promise for diagnosing LPS-induced neutrophils using MR imaging.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Immunology
Background:
- Neutrophils play a crucial role in the innate immune response.
- Lipopolysaccharide (LPS) induces neutrophil activation and phagocytic activity.
- Superparamagnetic iron oxide nanoparticles (SPIONs) offer potential for diagnostic imaging.
Purpose of the Study:
- To investigate the phagocytic activity of LPS-induced neutrophils labeled with mannan-coated SPIONs (M-SPIONs).
- To evaluate M-SPIONs as a tool for labeling and detecting LPS-induced neutrophils in vitro.
Main Methods:
- Human neutrophils were divided into control and LPS-induced groups.
- Neutrophils were labeled with M-SPIONs and dextran-coated SPIONs (D-SPIONs).
- Magnetic resonance imaging (MRI) signal intensity and intracellular iron uptake (Prussian blue staining) were assessed.
Main Results:
- M-SPION labeling resulted in significantly lower signal intensity in LPS-induced neutrophils compared to controls.
- Intracellular iron uptake was markedly demonstrated in LPS-induced neutrophils labeled with M-SPIONs.
- M-SPIONs proved more effective than D-SPIONs for in vitro neutrophil labeling.
Conclusions:
- In vitro labeling of LPS-induced neutrophils with M-SPIONs is feasible.
- This M-SPION labeling technique shows potential for developing a diagnostic method for LPS-induced neutrophils using MR imaging.
