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Updated: May 11, 2026
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Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
Published on: June 6, 2011
Metabolic glycan imaging by isonitrile-tetrazine click chemistry
Shaun Stairs1, André A Neves, Henning Stöckmann
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Chembiochem : a European Journal of Chemical Biology
|May 15, 2013
Abstract:
Seeing the sugar coating: N-Acetyl-glucosamine and mannosamine derivatives tagged with an isonitrile group are metabolically incorporated into cell-surface glycans and can be detected with a fluorescent tetrazine. This bioorthogonal isonitrile-tetrazine ligation is also orthogonal to the commonly used azide-cyclooctyne ligation, and so will allow simultaneous detection of the incorporation of two different sugars.
