Related Experiment Video
Updated: May 11, 2026
04:19
An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
Published on: March 10, 2023
Quantitative thin-layer chromatographic method for determination of amantadine hydrochloride
Hassan F Askal1, Alaa S Khedr, Ibrahim A Darwish
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Egypt;
International Journal of Biomedical Science : IJBS
|May 16, 2013
Summary
A new thin-layer chromatographic (TLC) method accurately quantifies amantadine hydrochloride (AMD). This simple, validated technique is stable under stress conditions and suitable for global laboratory use.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Amantadine hydrochloride (AMD) is an antiviral and antiparkinsonian agent.
- Accurate quantification methods are essential for pharmaceutical quality control.
Purpose of the Study:
- To develop and validate a simple, accurate, and robust thin-layer chromatographic (TLC) method for the quantitative determination of amantadine hydrochloride (AMD).
Main Methods:
- Quantitative determination using TLC on silica gel 60F-254 plates.
- Solvent system: n-hexane-methanol-diethylamine (80:40:5, v/v/v).
- Visualization via modified Dragendorff's reagent; detection by scanner.
Main Results:
- The method demonstrated linearity over 5-40 µg/spot (r=0.9994).
- Limits of detection (LOD) and quantitation (LOQ) were 0.72 and 2.38 µg/spot.
- AMD stability confirmed under various stress conditions (heat, acid/alkali, oxidation, UV).
- Method validated for accuracy, precision, and robustness, meeting ICH/FDA requirements.
- Successfully applied to bulk and capsule samples (99.0 ± 1.0% label claim).
Conclusions:
- The developed TLC method is simple, accurate, precise, and robust for AMD quantification.
- The method is cost-effective and suitable for widespread laboratory application.
- AMD is stable under investigated accelerated stress conditions.
Related Concept Videos
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
01:11Thin-Layer Chromatography (TLC): Overview
Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...