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Common excitatory synaptic inputs to electrically connected cortical fast-spiking cell networks.

Takeshi Otsuka1, Yasuo Kawaguchi

  • 1Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Aichi, Japan. otsuka@nips.ac.jp

Journal of Neurophysiology
|May 17, 2013
PubMed
Summary

Pyramidal cells locally regulate fast-spiking (FS) interneuron networks. Electrical connections between FS cells enhance activity spread, with effects depending on the network state and pyramidal cell input.

Keywords:
cortexfast-spiking cellgap junctionpyramidal cell

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Area of Science:

  • Neuroscience
  • Cellular Electrophysiology
  • Computational Neuroscience

Background:

  • Cortical fast-spiking (FS) interneurons possess extensive dendritic networks and electrical coupling via gap junctions.
  • These interneurons are crucial for cortical circuit function and information processing.
  • The regulatory mechanisms of FS interneuron network activity by other neuronal populations remain incompletely understood.

Purpose of the Study:

  • To investigate the role of pyramidal cells in modulating the activity of electrically coupled FS interneuron networks.
  • To determine how common inputs from pyramidal cells influence activity spread within FS interneuron networks.
  • To examine the dependence of this regulation on network state and the nature of synaptic connections.

Main Methods:

  • Paired recordings from cortical FS interneurons.
  • Glutamate puff stimulations to evoke synaptic inputs.
  • Experimental electrophysiology combined with computational simulations.

Main Results:

  • Electrically connected FS interneuron pairs share common inputs from pyramidal cells more frequently than chemically connected or unconnected pairs.
  • Common inputs to electrically connected FS cells enhance spike induction and induce inhibitory effects in the depolarized network state.
  • In the hyperpolarized state, common inputs induce depolarizing potentials in nearby FS cells, regardless of input strength.

Conclusions:

  • Pyramidal cells exert local, state-dependent regulation over globally connected FS interneuron networks.
  • Electrical connections between FS cells are critical for mediating activity spread influenced by pyramidal cell inputs.
  • The network state (depolarized vs. hyperpolarized) dictates the functional outcome of pyramidal cell input on FS interneuron network dynamics.