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Related Experiment Video

Updated: May 11, 2026

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
09:36

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Published on: June 23, 2022

Culturing astrocytes from postnatal rats.

Yangyang Huang1, Cheryl F Dreyfus

  • 1Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2013
PubMed
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Astrocyte cultures reveal novel functions and regulatory mechanisms. This chapter outlines the methodologies essential for establishing these informative cell cultures.

Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Astrocytes play crucial roles in brain function.
  • Understanding astrocyte regulation is vital for neuroscience research.

Purpose of the Study:

  • To detail methods for creating astrocyte cultures.
  • To highlight how cultures advance knowledge of astrocyte functions and regulation.

Main Methods:

  • Describes established protocols for astrocyte cell culture.
  • Focuses on techniques yielding reliable and informative cultures.

Main Results:

  • Astrocyte cultures have uncovered previously unknown functions.
  • These cultures provide insights into astrocyte regulatory pathways.

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Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
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Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Published on: October 21, 2017

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Last Updated: May 11, 2026

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
09:36

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Published on: June 23, 2022

Isolation and Culture of Mouse Cortical Astrocytes
11:25

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
15:08

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Published on: October 21, 2017

Conclusions:

  • Cell culture techniques are indispensable for advancing astrocyte biology.
  • Methodological details are key to successful astrocyte research.