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Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons
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Growth cone collapse assay.

Xin Yue1, Alexander I Son, Renping Zhou

  • 1Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2013
PubMed
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The growth cone collapse assay effectively detects axon guidance activities and aids in studying signaling pathways. This method helps identify compounds that promote axon regeneration, particularly for the repulsive molecule ephrin-A5.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Axon guidance is crucial for neural circuit formation.
  • Growth cone collapse assays are established methods for studying axon guidance.
  • Understanding repulsive axon guidance mechanisms is key for neural repair.

Purpose of the Study:

  • To describe a growth cone collapse assay protocol.
  • To investigate the signal transduction mechanisms of ephrin-A5.
  • To characterize the role of ephrin-A5 in repulsive axon guidance in hippocampal neurons.

Main Methods:

  • Utilizing a growth cone collapse assay.
  • Employing hippocampal neurons.
  • Studying the effects of the repulsive axon guidance molecule ephrin-A5.

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Related Experiment Videos

Last Updated: May 11, 2026

Visualizing Axonal Growth Cone Collapse and Early Amyloid &#946; Effects in Cultured Mouse Neurons
06:23

Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

Published on: October 30, 2018

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
07:53

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Published on: October 21, 2021

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
16:29

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

Published on: February 20, 2008

Main Results:

  • The growth cone collapse assay is effective for detecting and characterizing axon guidance activities.
  • The assay can dissect signaling pathways involving axon growth inhibitors.
  • The protocol facilitates the isolation of therapeutic compounds promoting axon regeneration.

Conclusions:

  • The growth cone collapse assay is a versatile tool in neuroscience research.
  • This assay protocol provides insights into ephrin-A5 signaling.
  • The method supports the development of strategies for promoting axon regeneration.