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Related Experiment Video

Updated: May 11, 2026

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
06:39

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

Published on: April 12, 2018

Neuroanatomical tract-tracing using biotinylated dextran amine.

Nikolai E Lazarov1

  • 1Department of Anatomy and Histology, Medical University-Sofia, Sofia, Bulgaria.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2013
PubMed
Summary

Biotinylated dextran amine (BDA) effectively traces nerve pathways. This study utilized BDA 10 kDa to reveal a direct, bilateral nigro-trigeminal pathway in rats, advancing neuroanatomical research.

Area of Science:

  • Neuroscience
  • Neuroanatomy
  • Cellular Biology

Background:

  • Biotinylated dextran amine (BDA) is a versatile tracer for neural pathway analysis.
  • BDA enables both light and electron microscopic visualization of neuronal connections.
  • It can be combined with other tracers or immunocytochemistry for detailed circuit mapping.

Purpose of the Study:

  • To describe an experimental protocol for anterograde and retrograde tracing using BDA.
  • To demonstrate the utility of BDA 10 kDa as an anterograde tracer.
  • To investigate the nigro-trigeminal pathway in rats.

Main Methods:

  • Application of Biotinylated Dextran Amine (BDA) 10 kDa as an anterograde tracer.
  • Utilizing BDA for tract-tracing in a rat model.

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Laser-guided Neuronal Tracing In Brain Explants
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Laser-guided Neuronal Tracing In Brain Explants

Published on: November 25, 2015

Related Experiment Videos

Last Updated: May 11, 2026

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
06:39

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

Published on: April 12, 2018

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
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Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Published on: September 14, 2015

Laser-guided Neuronal Tracing In Brain Explants
06:40

Laser-guided Neuronal Tracing In Brain Explants

Published on: November 25, 2015

  • Neuroanatomical analysis to map neuronal pathways.

    • Main Results:

    • Demonstration of a direct bilateral nigro-trigeminal pathway.
    • Successful application of BDA 10 kDa for anterograde tracing.
    • Validation of BDA's efficacy in mapping complex neuronal circuits.

    Conclusions:

    • BDA is a powerful tool for bidirectional neural pathway tracing.
    • A direct bilateral nigro-trigeminal pathway exists in rats.
    • The described protocol facilitates detailed neuroanatomical studies.