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Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar
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Technical note: a modified method to quantify prolamin proteins in dry and high-moisture corn.

S E Nellis1, P C Hoffman, R D Shaver

  • 1Department of Dairy Science, University of Wisconsin, Madison, Wisconsin 53706, USA.

Journal of Dairy Science
|May 21, 2013
PubMed
Summary

A modified rapid Bradford method (rBM) better quantifies functional prolamin proteins in high-moisture corn (HMC), which hinder starch digestibility. This method showed consistent results across dry and HMC, unlike the turbidimetric method.

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Area of Science:

  • Agricultural Science
  • Biochemistry
  • Animal Nutrition

Background:

  • Quantifying functional prolamin proteins, which impact starch digestibility, is crucial for animal feed.
  • Existing laboratory methods for prolamin quantification are less defined for high-moisture corn (HMC).

Purpose of the Study:

  • To modify a standard laboratory method for accurately quantifying functional prolamins in HMC.
  • To compare the efficacy of the modified method with a standard method in both dry and HMC.

Main Methods:

  • A rapid turbidimetric method (rTM) was modified into a rapid Bradford method (rBM) by incorporating a buffer-soluble protein extraction step.
  • Twenty samples of dry and HMC were analyzed using both rTM and rBM procedures.
  • In vitro gas production and peak absolute rates (PAR) were measured to assess the impact of prolamins on starch digestibility.

Main Results:

  • Both rTM and rBM methods yielded similar prolamin concentrations in dry corn.
  • The rBM method reported lower prolamin concentrations in HMC compared to the rTM method.
  • Prolamin concentrations measured by rTM showed variable relationships with PAR between dry and HMC.
  • Prolamin concentrations measured by rBM were negatively related to PAR, with no significant difference between corn types.

Conclusions:

  • The rapid Bradford method (rBM) provides a more consistent quantification of functional prolamin proteins that impede starch degradability in both dry and high-moisture corn.
  • The rBM method offers a more reliable approach for assessing the nutritional value of corn for animal feed.