Related Experiment Video
Updated: May 11, 2026

06:12
Production of Double-stranded DNA Ministrings
Published on: February 29, 2016
Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low
Ryohei Togashi1, Hidetaka Akita, Hideyoshi Harashima
1Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-Ku, Sapporo, Hokkaido 060-0812, Japan.
Journal of Bioscience and Bioengineering
|May 21, 2013
Abstract:
We report on the technical advance of linearized pDNA (pDNA(linear)) above the circular one (pDNA(circ)) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNA(linear) against poly-L-asparagine indicates that effective ion-pairing occurred between the pDNA(linear) and polycations.

