Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome reorganization and its functional impact during breast cancer progression.

eLife·2026
Same author

Connecting multiway enhancer-promoter interactions to changes in gene expression in cancer.

bioRxiv : the preprint server for biology·2026
Same author

Orderly mitosis shapes interphase genome architecture.

eLife·2026
Same author

TAD boundaries and gene activity are uncoupled.

eLife·2026
Same author

Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks.

bioRxiv : the preprint server for biology·2026
Same author

Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH.

STAR protocols·2026

Related Experiment Video

Updated: May 11, 2026

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems
09:29

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems

Published on: February 2, 2024

Non-coding RNAs in DNA damage and repair.

Vivek Sharma1, Tom Misteli

  • 1National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

FEBS Letters
|May 21, 2013
PubMed
Summary

Non-coding RNAs (ncRNAs) are vital for biological processes. This review highlights how ncRNAs are induced by DNA damage and regulate genome stability through cell cycle, apoptosis, and DNA repair mechanisms.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Non-coding RNAs (ncRNAs) are crucial regulators in various biological processes.
  • The DNA damage response (DDR) involves a complex signaling cascade upon DNA damage.
  • Emerging evidence points to ncRNAs as key components within the DDR.

Purpose of the Study:

  • To review the emerging role of ncRNAs in the context of DNA damage.
  • To summarize how ncRNAs are involved in the DNA damage response (DDR).
  • To highlight the function of ncRNAs in maintaining genome stability.

Main Methods:

  • Literature review of recent studies on ncRNAs and DNA damage.
  • Analysis of ncRNA induction following DNA damage.
  • Examination of ncRNA-mediated regulation of DDR pathways.

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

Related Experiment Videos

Last Updated: May 11, 2026

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems
09:29

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems

Published on: February 2, 2024

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

Main Results:

  • DNA damage induces the expression of various ncRNA species.
  • These ncRNAs play significant roles in regulating cell cycle progression.
  • ncRNAs are implicated in controlling apoptosis and DNA repair processes.

Conclusions:

  • ncRNAs are critical effectors in the DNA damage response.
  • ncRNAs contribute significantly to the maintenance of genome stability.
  • Further research into ncRNAs in DNA repair pathways is warranted.