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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
Characterisation of developmentally hypomineralised human enamel
Felicity A Crombie1, David J Manton, Joseph E A Palamara
1Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Australia.
Journal of Dentistry
|May 21, 2013
Summary
Molar incisor hypomineralisation (MIH) significantly alters enamel properties, reducing mineral content and hardness while increasing porosity and carbonate levels. These changes impact enamel structure and clinical presentation.
Area of Science:
- Dental Science
- Materials Science
- Biomineralization
Background:
- Molar incisor hypomineralisation (MIH) is a developmental defect affecting enamel quality.
- Understanding MIH's physical and chemical alterations is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate and clarify the physical and chemical properties of enamel affected by molar incisor hypomineralisation (MIH).
Main Methods:
- In vitro studies on extracted MIH-affected and sound molars.
- Vickers microhardness testing, transverse microradiography, polarized light microscopy.
- Carbonate content analysis and scanning electron microscopy (SEM).
Main Results:
- MIH enamel showed significantly reduced mineral content (58.8%vol%) and Vickers microhardness.
- Increased enamel porosity (up to 25%) and higher carbonate content (6.6 wt%) were observed in MIH.
- SEM revealed defective enamel rod and surface ultrastructure; cervical regions were spared.
Conclusions:
- MIH-affected enamel exhibits significantly different and variable properties compared to normal enamel.
- Observed alterations have implications for understanding MIH aetiology.
- Findings inform clinical management strategies for MIH-affected teeth.

