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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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A Protocol for Real-time 3D Single Particle Tracking
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Published on: January 3, 2018

Tracking single-particle dynamics via combined optical and electrical sensing.

Naoya Yukimoto1, Makusu Tsutsui, Yuhui He

  • 1The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

Scientific Reports
|May 21, 2013
PubMed
Summary

This study introduces a novel method combining electrical and optical sensing for tracking nanoscale particle motion. The technique achieves sub-MHz resolution, improving upon traditional fluorescent imaging for fast-moving features.

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Last Updated: May 11, 2026

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Published on: January 3, 2018

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Area of Science:

  • Physics
  • Nanotechnology
  • Biophysics

Background:

  • Fluorescent imaging is widely used for single-particle tracking.
  • Traditional wide-field microscopy lacks the temporal and spatial resolution for fast-moving features.

Purpose of the Study:

  • To develop a method for probing nanometer-scale motion of individual particles at sub-MHz resolution.
  • To overcome the limitations of fluorescent imaging for investigating fast-moving features.

Main Methods:

  • Combined resistive pulse technique with optical sensing.
  • Synchronously captured fluorescence images with ionic current measurements.
  • Analyzed spike-like electrical signals to discriminate translocation events.

Main Results:

  • Achieved sub-MHz resolution for probing nanometer-scale particle motion.
  • Demonstrated unambiguous discrimination between translocation and non-translocation events.
  • Observed trajectory-dependent translocation dynamics of voltage-driven single particles.

Conclusions:

  • The combined electrical/optical approach offers improved resolution for single-particle tracking.
  • This method can distinguish particle translocation events with high accuracy.
  • Potential applications exist in micro- and nano-electromechanical systems (MEMS/NEMS) sensor technologies.