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Updated: May 11, 2026
07:04
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
Published on: May 23, 2014
Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent
Rabeka Alam1, Joshua Zylstra, Danielle M Fontaine
1Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA.
Nanoscale
|May 21, 2013
Abstract:
Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.
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