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Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
Poliovirus: generation, quantification, propagation, purification, and storage
Cecily P Burrill1, Vanessa R Strings1, Raul Andino1
1Department of Microbiology and Immunology, University of California, San Francisco.
Current Protocols in Microbiology
|May 21, 2013
Summary
Poliovirus, a model RNA virus, can be cultured and studied using established protocols. These methods detail the production, propagation, quantification, and purification of poliovirus for research purposes.
Area of Science:
- Virology
- Molecular Biology
Background:
- Poliovirus (PV) is a non-enveloped, positive-sense RNA picornavirus.
- PV serves as a crucial model for studying RNA virus biology, pathogenesis, and evolution.
Purpose of the Study:
- To present established protocols for the production, propagation, quantification, and purification of poliovirus.
- To facilitate research on poliovirus biology and related RNA viruses.
Main Methods:
- Utilizing cDNA clones of various poliovirus strains.
- Producing infectious virus via transfection of in vitro transcribed viral genomes into host cells.
- Infecting and culturing human and non-human primate cell lines, such as HeLa and HeLa S3 cells.
Main Results:
- Poliovirus can be grown to high titers in susceptible cell lines.
- The presented protocols enable reliable generation and handling of infectious poliovirus.
Conclusions:
- Standardized protocols are essential for consistent and reproducible poliovirus research.
- These methods support ongoing investigations into RNA virus mechanisms and host interactions.
