Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

mTORC1 signaling requires proteasomal function and the involvement of CUL4-DDB1 ubiquitin E3 ligase.

Cell cycle (Georgetown, Tex.)·2008

Same author

Prospective study of liver transplant recipients with HCV infection: evidence for a causal relationship between HCV and insulin resistance.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·2008

Same author

Quantitative gel electrophoresis: sources of variation.

Journal of proteome research·2008

Same author

Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellular DSB repair.

Virology journal·2008

Same author

[Inhibitory effects of Qushi Huayu Decoction on fatty deposition and tumor necrosis factor alpha secretion in HepG2 cells induced by free fatty acid].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2008

Same author

Bioactive polybrominated diphenyl ethers from the marine sponge Dysidea sp.

Journal of natural products·2008

Search research articles

Related Experiment Video

Updated: May 11, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Glycomic analysis using glycoprotein immobilization for glycan extraction.

Shuang Yang¹, Yan Li, Punit Shah

¹Department of Pathology, Johns Hopkins University, Baltimore, MD 21231, USA.

Analytical Chemistry

|May 22, 2013

Summary

A new glycoprotein immobilization for glycan extraction (GIG) method simplifies complex glycomic analysis. This technique aids in disease diagnostics by enabling comprehensive study of N- and O-glycans.

More Related Videos

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Related Experiment Videos

Last Updated: May 11, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Area of Science:

Biochemistry
Analytical Chemistry
Molecular Biology

Background:

Glycosylation is a crucial post-translational modification affecting glycoprotein function.
Aberrant glycosylation patterns are linked to various diseases, making their study vital for diagnostics.
The inherent complexity of glycan biosynthesis poses challenges for comprehensive glycomic analysis.

Purpose of the Study:

To develop a novel and efficient method for comprehensive glycomic analysis.
To facilitate the extraction and analysis of glycans from complex biological and clinical samples.
To enable the study of both N- and O-glycans using a unified approach.

Main Methods:

Developed a method termed glycoprotein immobilization for glycan extraction (GIG).
Immobilized proteins/peptides from samples onto a solid support to remove contaminants.
Performed enzymatic or chemical modification of glycans on solid phase before release for mass spectrometry.

Main Results:

Successfully applied the GIG method to glycomic analysis.
Demonstrated the method's utility for analyzing both N-linked and O-linked glycans.
The GIG method allows for comprehensive analysis of complex glycan structures.

Conclusions:

The GIG method provides a robust approach for comprehensive glycomic profiling.
This technique can improve disease diagnostics by enabling detailed analysis of aberrant glycosylation.
The GIG method is applicable to diverse biological and clinical samples for glycan analysis.