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Updated: May 11, 2026
08:57
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format
Sofiène Abdellaoui1, Mourad Bekhouche, Alexandre Noiriel
1GEMBAS, ICBMS UMR 5246, Université Lyon 1, CNRS, INSA Lyon, CPE Lyon, 43 bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
Chemical Communications (Cambridge, England)
|May 22, 2013
Abstract:
The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.
