Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Artificial red cells. A link between the membrane skeleton and RES detectability?

R D MacGregor1, C A Hunt

  • 1School of Pharmacy, University of California, San Francisco 94143-0446.

Biomaterials, Artificial Cells, and Artificial Organs
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On the Meaning of the Term "Symptom".

Western journal of medicine and surgery·2024
Same author

Cold and Wet a Cause of Camp Disease, and Its Modus Operandi.

Chicago medical examiner·2023
Same author

Credibility, Replicability, and Reproducibility in Simulation for Biomedicine and Clinical Applications in Neuroscience.

Frontiers in neuroinformatics·2018
Same author

Phagedænic Ulcers in Assam.

The Indian medical gazette·2017
Same author

A Note on the Value of Revaccination.

The Indian medical gazette·2017
Same author

A Case of Cobra Bite-Recovery.

The Indian medical gazette·2017

Liposomes mimicking erythrocyte lipids are still detected by the reticuloendothelial system (RES). A membrane skeleton is crucial for reducing protein sorption and RES uptake, significantly extending circulation times.

Area of Science:

  • Biophysics
  • Biomaterials Science
  • Immunology

Background:

  • The reticuloendothelial system (RES) plays a key role in the clearance of foreign particles, but factors governing its detection of liposomes remain unclear.
  • Liposomes designed to mimic erythrocyte membranes show improved circulation times but are still rapidly cleared by the RES.
  • The absence of a membrane skeleton in current liposome formulations, such as Neohemocytes, is hypothesized to contribute to their RES detectability.

Purpose of the Study:

  • To investigate whether liposomes with erythrocyte outer leaflet lipid composition are invisible to the RES.
  • To explore the role of protein sorption and membrane skeletons in RES uptake of liposomes.
  • To develop a theoretical model quantifying protein binding to membranes and its effect on RES detectability.

Main Methods:

Related Experiment Videos

  • Development of a theoretical, statistical-thermodynamic model for protein binding to lipid membranes.
  • Analysis of factors influencing protein sorption, including membrane composition and the presence of a membrane skeleton.
  • Comparison of theoretical predictions with experimental data on liposome circulation times.

Main Results:

  • Liposomes mimicking erythrocyte lipid composition are not invisible to the RES, exhibiting circulation half-times significantly shorter than erythrocytes.
  • Protein sorption to lipid bilayers is identified as a critical factor in RES uptake.
  • The model predicts that a membrane skeleton can reduce protein sorption by several orders of magnitude, potentially by limiting the membrane area available for rapid lateral diffusion.

Conclusions:

  • The presence of a membrane skeleton is essential for minimizing protein sorption and, consequently, RES-mediated clearance of liposomes.
  • Avoidance of opsonization may be significantly influenced by the structural properties of a membrane skeleton.
  • A speculative model is proposed for RES detection of non-self lipid bilayers, highlighting the importance of membrane structure.