Related Experiment Video
Updated: May 11, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Controlling mammalian gene expression by allosteric hepatitis delta virus ribozymes
Yoko Nomura1, Linlin Zhou, Anh Miu
1Department of Biomedical Engineering, University of California, Davis , 451 Health Sciences Drive, Davis, California 95616, United States.
Abstract:
We engineered small molecule responsive allosteric ribozymes based on the genomic hepatitis delta virus (HDV) ribozyme by replacing the P4-L4 stem-loop with an RNA aptamer through a connector stem. When embedded in the 3' untranslated region of a reporter gene mRNA, these RNA devices enabled regulation of cis-gene expression by theophylline and guanine by up to 29.5-fold in mammalian cell culture. Furthermore, a NOR logic gate device was constructed by placing two engineered ribozymes in tandem, demonstrating the modularity of the RNA devices. The significant improvement in the regulatory dynamic range (ON/OFF ratio) of the RNA devices based on the HDV ribozyme should provide new opportunities for practical applications.
Related Concept Videos
Ribozymes
Ribozymes can be...
Ribozymes
Ribozymes can be...
Leaky Scanning
Viruses with RNA Genomes
Allosteric Regulation
Experimental RNAi

