Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Simple and Rapid Method for Preparing VHHAntibody-Template DNA Conjugates for Loop-Mediated Isothermal Amplification (LAMP) Method from cDNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Fluorogenic Peptide Aptamer Generated Using Bioorthogonal cDNA Display Technology for Cellular Imaging of Heat Shock Protein 90α.

Chembiochem : a European journal of chemical biology·2026
Same author

Harnessing the Power of SMART Single-Molecule Display for Enzyme Evolution: A Focus on Oxidase.

ACS synthetic biology·2026
Same author

A Novel Method for Preparing Uniform Micro-Sized Dry Powder Formulations, Including Aggregation-Controlled VHH.

Antibodies (Basel, Switzerland)·2025
Same author

Time- and sex-dependent effects of juvenile social isolation on mouse brain morphology.

NeuroImage·2025
Same author

Distinct neural responses of ventromedial prefrontal cortex-projecting nucleus reuniens neurons during aversive memory extinction.

Molecular brain·2025

Related Experiment Video

Updated: May 11, 2026

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Increasing the library size in cDNA display by optimizing purification procedures.

Yuki Mochizuki1, Shigefumi Kumachi, Koichi Nishigaki

  • 1Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan. nemoto@fms.saitama-u.ac.jp.

Biological Procedures Online
|May 24, 2013
PubMed
Summary
This summary is machine-generated.

This study enhances cDNA display yield for directed evolution. Optimized methods overcome ribosome interference and streamline purification, improving molecule yields tenfold for broader applications.

More Related Videos

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Related Experiment Videos

Last Updated: May 11, 2026

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biochemistry

Background:

  • Directed evolution requires large library sizes for effective molecular engineering.
  • cDNA display is a valuable in vitro display technology, but suffers from low yields.
  • Improving cDNA display yields is crucial for its practical application.

Purpose of the Study:

  • To optimize the cDNA display preparation process.
  • To identify and address factors limiting cDNA display molecule yield.
  • To enhance the utility of cDNA display in evolutionary molecular engineering.

Main Methods:

  • Systematic examination of each step in the cDNA display preparation protocol.
  • Investigating ribosome binding interference with biotin-streptavidin interactions.
  • Optimizing RNase digestion and purification steps, including buffer exchange reduction.

Main Results:

  • Identified steric hindrance from ribosomes as a key issue.
  • Demonstrated that performing RNase digestion in His-tag-binding buffer improves purification.
  • Achieved a more than 10-fold increase in cDNA display molecule yield.

Conclusions:

  • Optimized conditions significantly improve cDNA display yields.
  • The enhanced method makes cDNA display more accessible for directed evolution.
  • This advancement facilitates broader use of cDNA display in molecular engineering.