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Analysis of acyclovir in vitreous humor by a validated HPLC method
G R Silva1, A S P Caldeira, F M Damico
1School of Pharmacy, Federal University of São João Del, Divinópolis, Brazil. silvia.fialho@funed.mg.gov.br
Die Pharmazie
|May 25, 2013
Summary
A new HPLC-UV method accurately measures acyclovir in eye vitreous humor. This validated technique is suitable for analyzing acyclovir concentrations after direct ocular injection in rabbit eyes.
Area of Science:
- Analytical Chemistry
- Ophthalmology
- Pharmacokinetics
Background:
- Acyclovir is a crucial antiviral medication used to treat ocular infections.
- Accurate quantification of acyclovir in ocular tissues is essential for understanding its efficacy and safety.
- Existing methods may lack the sensitivity or specificity required for vitreous humor analysis.
Purpose of the Study:
- To develop and validate a High-Performance Liquid Chromatography with Ultraviolet detection (HPLC-UV) method for determining acyclovir concentrations in vitreous humor.
- To establish a reliable analytical tool for pharmacokinetic studies of acyclovir in ocular tissues.
Main Methods:
- Development of an isocratic HPLC-UV method utilizing a C18 column.
- Mobile phase composition: 0.02 mol/L acetic acid/methanol (95:5).
- UV detection at 254 nm, column temperature maintained at 25°C.
Main Results:
- The method demonstrated excellent linearity (r² > 0.99) within the 35-700 µg/mL range.
- High precision (RSD <5%) and accuracy (98.18-99.64% recovery) were achieved.
- The method proved to be robust and selective for vitreous humor matrices.
Conclusions:
- The validated HPLC-UV method is suitable for the direct determination of acyclovir in rabbit vitreous humor.
- This analytical method can support research on the ocular pharmacokinetics of acyclovir.
