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[Influence of nuclear factor-kappa B inhibition on -SH groups concentration in liver of rats]
Łukasz Michalski1, Paulina Kleniewska, Aleksandra Piechota-Polańczyk
1Department of Cardiovascular Physiology, Medical University of Lodz, Poland.
The Aim Of The Study:
was to evaluate the influence of nuclear factor-kappaB inhibition on -SH groups concentration in liver of rat
Material And Methods:
Experiments were performed on Wistar-Kyoto rats divided into following groups: group I (control) received saline; group II (ET-1) received endothelin 1 (12.5 microg/kg b.w.); Group III (BAY 11-7082) received a nuclear factor-kappaB (NF-kappaB) inhibitor (10 mg/kg b.w.); Group IV (BAY 11-7082+ET-1) received a nuclear factor-kappa B inhibitor (10 mg/kg b.w.) and 30 min later ET-1 (12.5 microg/kg b.w.).
Results:
Administration of endothelin 1 (ET-1) at a dose 12.5 microg/kg b.w. resulted in a statistically significant decrease in the concentration of -SH groups (p < 0.05 vs control). In contrast, BAY11-7082 (10 mg/kg b.w.) significantly increased the level of -SH groups in the liver in ET-1 inquced oxidative stress (p < 0.02).
Conclusion:
A nuclear factor-kappa B inhibitor (BAY 11-7082) at a dose 10 mg/kg b.w. showed a significant increase in the concentration of -SH groups in the liver, in ET-1 induced oxidative stress.
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