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Purification, characterization and preparation immunomatrixes of S-layer proteins of Thermobifida fusca
S Pervaiz1, T Shaheen, S Shaheen
1Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan.
Aim:
S-layer proteins are considered as a good nanocarrier due to their binding and self-assembled properties. These can be used to prepare the immunomatrixes for the removal of toxins from the samples.
Methods And Results:
Two S-layer proteins 70 and 40 kDa of thermophilic Thermobifida fusca were extracted with guanidine hydrochloride and purified. Antibodies against S-layer proteins were developed, and their monospecificity was checked. Immunogold labelling indicated that these are surface proteins. Immunomatrixes (70-SLIM, 40 SLIM) were prepared by covalently immobilizing S-layer proteins in microwell and further conjugated with anti- Staphylococcus aureus enterotoxin B (SEB) antibodies. The binding of 70 and 40 kDa proteins was observed nearly 7·0 μg cm(-1) to binding area, and the conjugation with anti-SEB antibodies was found 1·22 μg μg(-1) of 70 kDa and 0·875 μg μg(-1) of 40 kDa. The average binding and elution of pure SEB toxin on 70-SLIM and 40-SLIM was 5·0 μg SEB toxin. The SEB toxin in milk samples was also removed on immunomatrixes successfully.
Conclusion:
It is the first report, and this study shows that the thermophilic S-layer proteins can be used to prepare the immunomatrixes.
Significance And Impact Of Study:
Information in this study can be used to design the strategies for the removal of biologically important materials or toxins from samples.
