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Updated: May 11, 2026
09:39
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
Cell biology: DUBing CP110 controls centrosome numbers
Felix Bärenz1, Ingrid Hoffmann
1Cell Cycle Control and Carcinogenesis, F045, German Cancer Research Center, DKFZ, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany.
Current Biology : CB
|May 25, 2013
Summary
The deubiquitinating enzyme USP33 is crucial for controlling centrosome number. USP33 stabilizes the CP110 protein, ensuring proper centrosome biogenesis and genomic stability.
Area of Science:
- Cell Biology
- Genomics
- Biochemistry
Background:
- Centrosome number must be tightly regulated for genomic integrity.
- Dysregulation of centrosome number is linked to diseases like cancer.
Purpose of the Study:
- To investigate the role of the deubiquitinating enzyme USP33 in centrosome biogenesis.
- To elucidate the mechanism by which USP33 controls centrosome duplication.
Main Methods:
- Utilized cell-based assays to examine USP33 function.
- Investigated the interaction between USP33 and the centriolar protein CP110.
- Assessed the impact of USP33 on centrosome duplication and stability.
Main Results:
- USP33 was found to regulate centrosome biogenesis.
- USP33 stabilizes the centriolar protein CP110.
- USP33 activity is essential for maintaining correct centrosome numbers.
Conclusions:
- USP33 plays a critical role in controlling centrosome number through CP110 stabilization.
- This mechanism is vital for preserving genomic integrity.
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