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Related Experiment Video

Updated: May 11, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

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Published on: December 19, 2018

Interaction between IGF-IR and ER induced by E2 and IGF-I.

Zhenghong Yu¹, Weimin Gao, Enze Jiang

¹Department of Medical Oncology, Jinling Hospital, Nanjing, China.

|May 25, 2013

Summary

Estrogen receptor (ER) interacts with the insulin-like growth factor-I receptor (IGF-IR), influencing breast cancer cell growth. This interaction is crucial for ER

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Published on: December 3, 2014

Area of Science:

Molecular Biology
Endocrinology
Cancer Research

Background:

Estrogen receptor (ER) and insulin-like growth factor-I receptor (IGF-IR) play roles in breast cancer cell proliferation.
Estrogen and IGF-I exhibit synergistic effects on breast cancer growth.
Non-nuclear, non-genomic effects of ER are an emerging area of research.

Purpose of the Study:

To investigate the mechanism behind the synergistic effects of estrogen and IGF-I on breast cancer cells.
To elucidate the role of ER-IGF-IR interaction in non-genomic ER signaling.

Main Methods:

Utilized MCF-7 breast cancer cell lines with reduced IGF-IR expression (MCF-7(SX13)).
Employed NIH3T3 cells overexpressing IGF-IR (NWTB3) transiently transfected with ERα.
Assessed ER-IGF-IR association, ER translocation, and downstream signaling pathway phosphorylation (ERK1/2, Akt).

Main Results:

Estradiol (E2) and IGF-I induced ER-IGF-IR association in MCF-7 cells, which was lost in MCF-7(SX13) cells.
ERα and IGF-IR interaction was induced by E2 and IGF-I in NWTB3 cells.
ERα modulated IGF-I signaling via ERK1/2 and Akt phosphorylation; ER-IGF-IR interaction enhanced cell growth and ER translocation to the cytoplasm.

Conclusions:

The interaction between ER and IGF-IR is essential for the non-genomic effects of ER.
ER-IGF-IR complex formation potentiates breast cancer cell growth and influences signaling pathways.