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Published on: November 11, 2022
[Study on the fingerprint of Morus alba from different habitats by HPLC]
Cheng Chen1, Hong-Bo Li, Liu-Ping Wang
1Guangxi University of Chinese Medicine, Nanning 530001, China. ccjhs2005112@163.com
Objective:
To establish HPLC fingerprint of Morus alba from different habitats by HPLC and provide basis for its quality control.
Methods:
HPLC analysis was performed on an Agilent XDB C18 Column (4.6 mm x 250 mm, 5 microm), gradient eluted composed of acetonitrile and 0.3% phosphate acid. The column temperature was set at 35 degrees C and the flow rate was 0.5 mL/min. The detective wavelength was 290 nm. The HPLC fingerprint for 10 batches of Morus alba was studied on their similarity.
Results:
There were twelve common peaks in the fingerprint. The similarity of 7 batches was above 0.9 and the other batches had low similarity.
Conclusion:
The HPLC fingerprint can be used for quality control of Morus alba with high characteristics and specificity.
