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Updated: May 11, 2026

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
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Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Precise quantification of nanoparticle internalization.

Claudia Gottstein1, Guohui Wu, Benjamin J Wong

  • 1Department of Chemical Engineering and California NanoSystems Institute, University of California-Santa Barbara, Santa Barbara, California 93106-6105, USA. gottstein@lifesci.ucsb.edu

ACS Nano
|May 28, 2013
PubMed
Summary
This summary is machine-generated.

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A new mathematical model quantifies nanoparticle uptake into cells, distinguishing between associated and internalized particles. This method, applied to vesosome coatings, suggests polyethylene glycol may reduce rapid clearance by reticuloendothelial system cells.

Area of Science:

  • Biomedical Nanotechnology
  • Cellular Biology
  • Drug Delivery Systems

Background:

  • Nanoparticles offer promising diagnostic and therapeutic applications.
  • Accurate quantification of nanoparticle internalization is crucial for drug delivery.
  • Existing methods lack standardization and struggle to differentiate associated from internalized particles.

Purpose of the Study:

  • To develop a standardized, quantitative assay for measuring nanoparticle internalization.
  • To differentiate between nanoparticle association and internalization.
  • To assess the impact of surface coatings on nanoparticle uptake by reticuloendothelial system (RES) cells.

Main Methods:

  • Developed a mathematical model integrating flow cytometry and confocal microscopy data.

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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

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Last Updated: May 11, 2026

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
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Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation
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Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

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14:53

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  • Applied the model to study vesosome nanoparticle uptake.
  • Investigated the effect of long-chain polyethylene glycol coating on vesosomes.

    • Main Results:

    • The developed method provides a quantitative and potentially high-throughput approach for measuring nanoparticle internalization.
    • The study observed a trend indicating that long-chain polyethylene glycol coating on vesosomes may reduce their internalization by RES cells.
    • This finding is significant as RES cells are responsible for rapid nanoparticle clearance.

    Conclusions:

    • The novel mathematical model offers a standardized tool for biomedical nanotechnology research.
    • Understanding and controlling nanoparticle uptake by RES cells is key to improving nanoparticle-based therapies.
    • Surface modification strategies, like PEGylation, warrant further investigation to enhance nanoparticle circulation times.