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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

Microbe-Plant Interactions

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...

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Related Experiment Video

Updated: May 11, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Plant microRNAs and development.

¹The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Sciences, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China. wugang@zafu.edu.cn

Journal of Genetics and Genomics = Yi Chuan Xue Bao

|May 28, 2013

Summary

This summary is machine-generated.

This review explores microRNAs (miRNAs), small non-coding RNAs regulating plant development. It details miRNA biogenesis, effector pathways, and their roles in plant growth, highlighting recent advances.

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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Area of Science:

Plant molecular biology
Genetics and genomics
Biochemistry

Background:

MicroRNAs (miRNAs) are small, non-coding RNA molecules (~20-24 nucleotides) crucial for gene expression regulation.
Both transcriptional and post-transcriptional gene silencing mechanisms are influenced by miRNAs.
Recent research has uncovered novel components and regulatory pathways in miRNA biogenesis and function.

Purpose of the Study:

To review the key components of the miRNA biogenesis and effector pathways in plants.
To discuss developmental defects linked to mutations in these pathways.
To highlight the functions and modes of action of important plant miRNAs.

Main Methods:

Literature review of recent studies on plant miRNA pathways.
Analysis of genetic mutations affecting miRNA biogenesis and function.
Synthesis of current knowledge on miRNA roles in plant development.

Main Results:

Identification of essential components in miRNA biogenesis and effector pathways.
Correlation between mutations in these pathways and observable developmental defects in plants.
Elucidation of specific miRNA functions and their regulatory mechanisms in plant growth.

Conclusions:

The miRNA pathway is fundamental to plant development.
Understanding miRNA components and their regulation is key to addressing developmental defects.
This review provides a comprehensive overview of recent advancements in plant miRNA research.