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Updated: May 11, 2026
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Published on: May 1, 2019
Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery
Luis V Rodríguez Durán1, Darío Spelzini, Valeria Boeris
1Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza and J. Cárdenas s/n, ZIP 25280, Saltillo, Coahuila, Mexico.
Abstract:
The interaction of tannase (TAH) with chitosan, polyethyleneimine and Eudragit(®)E100 was studied. It was found that TAH selectively binds to these polycations (PC), probably due to the acid nature of the target protein. TAH could interact with these PC depending on the medium conditions. The effect of the interaction on the secondary and tertiary structure of TAH was assayed through circular dichroism and fluorescence spectroscopy. TAH was recovered from Aspergillus niger culture broth by means of precipitation and adsorption using chitosan.
