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Related Experiment Video

Updated: May 11, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Selecting agonists from single cells infected with combinatorial antibody libraries.

Hongkai Zhang1, Kyungmoo Yea, Jia Xie

  • 1Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Chemistry & Biology
|May 28, 2013
PubMed
Summary

Researchers developed a novel system for selecting receptor agonist antibodies. This method uses lentiviruses and fluorescent reporter cells to directly link antibody genes to their function, enabling rapid discovery of potent agonists.

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Last Updated: May 11, 2026

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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Area of Science:

  • Biotechnology
  • Immunology
  • Molecular Biology

Background:

  • Developing receptor agonists is crucial for therapeutic applications.
  • Existing methods for antibody selection can be inefficient and time-consuming.

Purpose of the Study:

  • To present a novel system for the direct selection of receptor agonist antibodies.
  • To establish a method for linking antibody genotype to phenotype for efficient screening.

Main Methods:

  • Utilizing combinatorial antibody libraries delivered via lentiviruses.
  • Infecting eukaryotic cells engineered with a receptor-coupled fluorescent reporter system.
  • Employing a co-replication strategy to link lentivirus genotype with cell phenotype.
  • Sorting fluorescent cells to isolate positive clones and recover antibody genes.

Main Results:

  • Successfully generated potent antibody agonists.
  • Demonstrated the system's ability to create thrombopoietin (TPO) phenocopies rapidly.
  • Validated the direct link between genotype and phenotype for antibody selection.

Conclusions:

  • The described system enables efficient and direct selection of functional receptor agonist antibodies.
  • The methodology is potentially generalizable to various signaling pathways with selectable phenotypes.
  • This approach accelerates the discovery of novel antibody therapeutics.