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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Published on: November 4, 2021
Fast mass spectrometry detection of tryptophan-containing peptides and proteins by reduction with pyridine-borane
1Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel.
Analytical Biochemistry
|May 28, 2013
Abstract:
In this note, we describe a method devised to detect, by means of mass spectrometry (MS), tryptophan-containing peptides and proteins using pyridine-borane. This reagent selectively reduces tryptophan residues, converting them to 2,3-dihydro-tryptophan, thereby enabling quantitation of tryptophans.
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