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Updated: May 11, 2026

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Published on: March 23, 2020
PIUS: peptide identification by unbiased search
Eduardo P Costa1, Gerben Menschaert, Walter Luyten
1Department of Computer Science, KU Leuven, Celestijnenlaan 200A, B-3001 Heverlee, Belgium. eduardo.costa@cs.kuleuven.be
Summary:
We present PIUS, a tool that identifies peptides from tandem mass spectrometry data by analyzing the six-frame translation of a complete genome. It differs from earlier studies that have performed such a genomic search in two ways: (i) it considers a larger search space and (ii) it is designed for natural peptide identification rather than proteomics. Differently from other peptidomics tools designed for genome-wide searches, PIUS does not limit the analysis to a set of sequences that match a list of de novo reconstructions.
Availability:
Source code, executables and a detailed technical report are freely available at http://dtai.cs.kuleuven.be/ml/systems/pius.
Contact:
eduardo.costa@cs.kuleuven.be
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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