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Updated: May 11, 2026

Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa
Published on: March 15, 2024
Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
Theerthankar Das1, Mike Manefield
1Centre for Marine BioInnovation (CMB); School of Biotechnology and Biomolecular Sciences (BABS); University of New South Wales (UNSW); Sydney, NSW Australia.
Abstract:
In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated significantly less eDNA in comparison with the phenazine producing strains. The relationship between eDNA release and phenazine production is bridged via hydrogen peroxide (H2O2) generation and subsequent H2O2 mediated cell lysis and ultimately release of chromosomal DNA into the extracellular environment as eDNA.
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