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Related Experiment Video

Updated: May 11, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Molecular techniques for dicistrovirus detection without RNA extraction or purification.

Jailson F B Querido¹, Jon Agirre, Gerardo A Marti

¹Centre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal.

Biomed Research International

|May 28, 2013

Summary

Researchers developed a rapid method to detect Triatoma virus (TrV), a pathogen affecting Chagas disease vectors. This sensitive technique identifies TrV in fecal samples without needing viral RNA extraction.

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Area of Science:

Virology
Entomology
Vector-borne disease research

Background:

Dicistroviridae are small, nonenveloped, positive-sense single-stranded RNA viruses impacting arthropods.
Triatoma virus (TrV) infects Triatoma infestans, a primary vector for Chagas disease.
Accurate identification of TrV is crucial for understanding its role in vector populations.

Purpose of the Study:

To develop and validate a simple, single-step method for identifying Triatoma virus (TrV).
To assess the sensitivity of the identification method for TrV in triatomine fecal samples.
To provide a tool for rapid detection of TrV without prior RNA extraction.

Main Methods:

A single-step protocol was established for TrV identification.
The method was applied to fecal samples collected from triatomines.
Viral RNA extraction and purification steps were omitted to streamline the process.

Main Results:

The developed method successfully identified TrV in triatomine fecal samples.
The identification technique demonstrated high sensitivity.
Effective TrV detection was achieved without the need for RNA extraction and purification.

Conclusions:

A rapid and sensitive single-step method for TrV identification has been established.
This method simplifies TrV detection from fecal samples, bypassing RNA isolation.
The findings offer a valuable tool for epidemiological studies and vector control strategies targeting Chagas disease.