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Updated: May 11, 2026

Introduction to Solid Supported Membrane Based Electrophysiology

Introduction to Solid Supported Membrane Based Electrophysiology

Published on: May 11, 2013

Introduction to solid supported membrane based electrophysiology.

Andre Bazzone¹, Wagner Steuer Costa, Markus Braner

¹Department of Biophysical Chemistry, Max Planck Institute of Biophysics. andre.bazzone@biophys.mpg.de

Journal of Visualized Experiments : Jove

|May 29, 2013

Summary

We developed a novel electrophysiological method using solid supported membranes to study membrane protein transport. This technique measures transient currents to reveal transporter kinetics, offering a new approach for challenging membrane proteins.

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Preparation of Artificial Bilayers for Electrophysiology Experiments

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Published on: October 31, 2008

Area of Science:

Biophysics
Membrane Protein Research
Electrophysiology

Background:

Studying membrane protein function is crucial for understanding cellular processes.
Existing electrophysiological methods like patch clamp have limitations for certain membrane proteins.

Purpose of the Study:

To present a novel electrophysiological method for investigating membrane protein transport.
To enable the study of prokaryotic and intracellular eukaryotic membrane transporters.

Main Methods:

Utilized a solid supported membrane (SSM) with a gold-coated sensor chip and phosphatidylcholine monolayer.
Employed a cuvette system with a reference electrode for electrophysiological measurements.
Induced protein activity via fast solution exchange and measured transient currents using capacitive coupling.

Main Results:

The method successfully measures electrogenic transport activity of membrane proteins.
Transient currents provide kinetic information, with peak current reflecting stationary activity.
Time-dependent transporter currents can be reconstructed through circuit analysis.

Conclusions:

This SSM-based electrophysiological method offers a viable alternative for studying membrane proteins.
It is particularly suitable for transporters not amenable to traditional patch or voltage clamp techniques.