Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Large-area low-noise resonant photodetector for high-sensitivity stimulated Raman scattering spectroscopy and imaging.

Optics express·2026
Same author

Self-Calibrated Stimulated Raman Scattering Spectroscopy for Rapid Cholangiocarcinoma Diagnosis.

Analytical chemistry·2025
Same author

Merging Vibrational Spectroscopy with Fluorescence Microscopy: Combining the Best of Two Worlds.

Annual review of physical chemistry·2025
Same author

Transient stimulated Raman scattering spectroscopy and imaging.

Light, science & applications·2024
Same author

Transient Stimulated Raman Excited Fluorescence Spectroscopy.

Journal of the American Chemical Society·2023
Same author

SeHed, a novel gene expression system with stress-evoked hydrogen peroxide elimination property and anti-aging effect.

Signal transduction and targeted therapy·2022

Related Experiment Video

Updated: May 11, 2026

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
04:35

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection.

Xiaoli Qi1, Hanqing Xiong, Xiaohua Lv

  • 1Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan 430074, China.

Journal of Biomedical Optics
|May 30, 2013
PubMed
Summary

This study introduces confocal detection to improve neuronal circuit tracing in large brain volumes. This method enhances signal detection, enabling continuous fiber tracing despite sectioning-induced interruptions.

More Related Videos

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
09:40

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

Related Experiment Videos

Last Updated: May 11, 2026

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
04:35

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
09:40

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

Area of Science:

  • Neuroscience
  • Optical Imaging
  • Microscopy

Background:

  • Micro-optical sectioning tomography enables submicron resolution imaging of large brain volumes.
  • Sectioning fluorescence samples can cause tears, interrupting continuous neuronal fiber tracing.
  • Challenges exist in maintaining signal integrity during large-scale neuronal circuit mapping.

Purpose of the Study:

  • To introduce a confocal detection method to overcome interruptions in fluorescence imaging during micro-optical sectioning tomography.
  • To enhance the detectability of neuronal signals in damaged or interrupted sections.
  • To enable continuous tracing of neuronal circuits in large brain volumes.

Main Methods:

  • Integration of a confocal detection system with a micro-optical sectioning tomography setup.
  • Utilizing a 50-μm-width confocal slit during optical imaging.
  • Quantifying the signal-to-background ratio improvement with and without the confocal slit.

Main Results:

  • The confocal detection with a 50-μm slit significantly increased the signal-to-background ratio by 16- to 49-fold.
  • Improved signal detectability in interruptions caused by sample sectioning.
  • Facilitated continuous tracing of neuronal fibers and circuits.

Conclusions:

  • Confocal detection effectively recovers interruptions in fluorescence imaging during micro-optical sectioning tomography.
  • This technique enhances the feasibility of mapping large-scale neuronal circuits with improved continuity.
  • The method offers a robust solution for overcoming artifacts in high-resolution brain circuit reconstruction.