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Related Concept Videos

Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

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Related Experiment Video

Updated: May 11, 2026

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

Fibronectin mediates mesendodermal cell fate decisions.

Paul Cheng1, Peter Andersen, David Hassel

  • 1Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA.

Development (Cambridge, England)
|May 30, 2013
PubMed
Summary
This summary is machine-generated.

Endodermal cells promote mesoderm formation via short-range signals, including fibronectin. This interaction regulates Wnt signaling through integrin-β1, crucial for embryonic development.

Keywords:
EndodermFibronectinIntegrin-β1MesodermWnt

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TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
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Last Updated: May 11, 2026

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TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
07:05

TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Published on: February 26, 2021

Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Extracellular Matrix Biology

Background:

  • Non-cell-autonomous signals are critical for embryonic cell fate decisions.
  • Reciprocal endoderm-mesoderm signaling is vital but poorly understood.
  • Key signals and mechanisms governing this interaction require elucidation.

Purpose of the Study:

  • To investigate the role of endoderm-derived signals in promoting mesoderm emergence.
  • To identify the specific molecules and pathways involved in endoderm-mesoderm interactions.
  • To elucidate the function of fibronectin in mediating these developmental processes.

Main Methods:

  • Utilized mouse embryonic stem cells to study endoderm-mesoderm interactions and precardiac mesoderm formation.
  • Investigated the role of endodermal fibronectin production in promoting mesoderm development.
  • Employed zebrafish embryos to assess the in vivo effects of fibronectin deficiency on mesoderm and endoderm development.
  • Analyzed the involvement of Wnt signaling and integrin-β1 activation in fibronectin-mediated mesoderm induction.

Main Results:

  • Endodermal cells were shown to promote mesoderm emergence through short-range signals.
  • Endoderm-mesoderm interaction, involving endodermal fibronectin, promoted precardiac mesoderm formation in vitro.
  • Fibronectin deficiency in zebrafish led to reduced mesoderm and expanded endoderm.
  • This process was mediated by fibronectin regulating Wnt signaling in mesodermal cells via integrin-β1 activation.

Conclusions:

  • Endoderm plays a crucial role in promoting mesoderm emergence through short-range signaling.
  • Fibronectin, an extracellular matrix component, is a key mediator of this endoderm-mesoderm interaction.
  • Integrin-β1-mediated regulation of Wnt signaling is a novel downstream pathway for fibronectin in mesoderm induction.