Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rashba-Related Spin-Selective Effect in 2D Chiral Perovskites with Achiral Organic Cation Spacers.

Nano letters·2026
Same author

Case Report: Left ventricular hemorrhage associated with early neonatal mortality in multi-gene engineered cloned pigs.

Frontiers in veterinary science·2026
Same author

Antagonistic regulation of anthocyanin biosynthesis by HY5 and BPC1 in <i>Arabidopsis thaliana</i>.

Frontiers in plant science·2026
Same author

Single-cell multi-omics analysis reveals two molecular subtypes of human male breast cancer with distinct neuroendocrine and immune characteristics.

Journal of advanced research·2026
Same author

Epigenetically regulated transcriptional repressor, BASIC PENTACYSTEINE1 (BPC1) acts together with polycomb repressive complex 2 (PRC2) to suppress camalexin biosynthesis in Arabidopsis.

Plant molecular biology·2026
Same author

Imparting Biodegradability to Highly-Efficient Upconversion Nanoparticles via Facet-Selective Zirconium Doping.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

DNAzyme-Enhanced CRISPR/Cas12a Cascade Enables Isothermal, One-Pot RNA Diagnostics.

ACS applied materials & interfaces·2026
Same journal

Continuous π-Conjugation in β-Ketoenamine Covalent Organic Frameworks Boosts Charge Transfer for Selective Photocatalysis.

ACS applied materials & interfaces·2026
Same journal

Scalable Ionogel-Based Thermochromic Smart Windows: Enhanced Solar Regulation, Weatherability, and Processability.

ACS applied materials & interfaces·2026
Same journal

Metal-Organic Framework Monoliths Derived from Emulsion-Templated Foams for Reactive Filtration.

ACS applied materials & interfaces·2026
Same journal

Binary to Quaternary Rare-Earth Phosphates: Compositional Effects on Thermal Properties and CMAS Corrosion Resistance of Environmental Barrier Coatings.

ACS applied materials & interfaces·2026
Same journal

Suture-Free Piezoelectric Band-Aid Membrane for Complex Peripheral Nerve Defects.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: May 11, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Fluorescent pH sensor based on Ag@SiO2 core-shell nanoparticle.

Zhenhua Bai1, Rui Chen, Peng Si

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore.

ACS Applied Materials & Interfaces
|May 30, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fluorescence pH sensor using silver nanoparticles and a pH-sensitive dye. This sensor shows enhanced fluorescence and sensitivity for real-time biological pH monitoring.

More Related Videos

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
07:13

Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

Published on: November 15, 2016

Related Experiment Videos

Last Updated: May 11, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
07:13

Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

Published on: November 15, 2016

Area of Science:

  • Nanotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Developing sensitive and reliable pH sensors is crucial for biological and chemical applications.
  • Existing sensors often face limitations in sensitivity, real-time monitoring, or applicability in biological samples.

Purpose of the Study:

  • To create a novel fluorescence-based pH sensor with enhanced sensitivity and potential for real-time biological monitoring.
  • To optimize the sensor design by incorporating a silica shell between a silver core and a pH-sensitive dye.

Main Methods:

  • Fabrication of a silver (Ag) core nanoparticle coated with a thickness-variable silica (SiO2) shell.
  • Incorporation of a pH-sensitive dye (HPTS) onto the silica shell.
  • Characterization of fluorescence enhancement and pH sensitivity using spectrophotometry.

Main Results:

  • Maximum fluorescence enhancement was achieved with an 8 nm silica shell thickness.
  • Fluorescence intensity increased 4-fold and 9-fold upon excitation at 405 nm and 455 nm, respectively.
  • The sensor demonstrated good pH sensitivity in the range of 5-9, with a determined emission intensity ratio versus pH.

Conclusions:

  • The developed Ag core-SiO2 shell-HPTS system represents a novel and effective fluorescence-based pH sensor.
  • The sensor exhibits significant fluorescence enhancement and pH sensitivity, making it suitable for biological applications.
  • This sensor holds potential for real-time pH determination in biological samples.