Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Essential Minerals for Bone Health01:31

Essential Minerals for Bone Health

The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
Masking and Demasking Agents01:19

Masking and Demasking Agents

EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on the metal...
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants01:18

Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants

Oral anticoagulants are vital tools in preventing and treating blood clotting disorders. This diverse class of medications can be categorized as vitamin K antagonists, exemplified by warfarin, and direct thrombin inhibitors (DTIs), such as dabigatran, as well as factor Xa inhibitors, including rivaroxaban.
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Type IV collagen is a novel DEJ biomarker that is reduced by radiotherapy.

Journal of dental research·2014
Same author

Addition of Grape Seed Extract Renders Phosphoric Acid a Collagen-stabilizing Etchant.

Journal of dental research·2014
Same author

DMP1-targeted Cre expression in odontoblasts and osteocytes.

Journal of dental research·2007
Same author

Mechanism by which MLO-A5 late osteoblasts/early osteocytes mineralize in culture: similarities with mineralization of lamellar bone.

Calcified tissue international·2006
Same author

DMP1 is essential for osteocyte formation and function.

Journal of musculoskeletal & neuronal interactions·2005
Same author

Anti-B4-blocked ricin synergizes with doxorubicin and etoposide on multidrug-resistant and drug-sensitive tumors.

Blood·1995

Related Experiment Video

Updated: May 10, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
08:26

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

Proanthocyanidins rapidly stabilize the demineralized dentin layer.

Y Liu1, V Dusevich, Y Wang

  • 1University of Missouri-Kansas City School of Dentistry, 650 E 25th Street, Kansas City, MO 64108, USA.

Journal of Dental Research
|June 1, 2013
PubMed
Summary

Proanthocyanidins (PA) effectively stabilize demineralized dentin collagen against enzymatic degradation. Applied as a primer, PA offers superior protection compared to glutaraldehyde in clinically relevant timeframes.

Keywords:
collagencollagenasecross-linking reagentsdental acid etchingscanning electron microscopytransmission electron microscopy

More Related Videos

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Related Experiment Videos

Last Updated: May 10, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
08:26

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Area of Science:

  • Biomaterials Science
  • Dental Materials
  • Biochemistry

Background:

  • Proanthocyanidins (PA) enhance collagen resistance to degradation.
  • Direct incorporation of PA into adhesive systems hinders light-curing.
  • PA as a primer may overcome limitations, but clinical efficacy is underexplored.

Purpose of the Study:

  • To investigate the efficacy of PA in stabilizing acid-etched dentin collagen.
  • To evaluate PA's performance when applied as a primer in clinically relevant short timeframes.
  • To compare PA's stabilizing effect with glutaraldehyde.

Main Methods:

  • Acid-etched dentin collagen was treated with PA for brief periods.
  • Morphology was assessed before and after collagenase digestion.
  • Comparison with untreated controls and glutaraldehyde-treated samples.

Main Results:

  • Untreated collagen degraded within 1 hour of digestion.
  • PA-treated collagen remained stable for at least 16 hours.
  • Glutaraldehyde showed minimal stabilizing effect under tested conditions.

Conclusions:

  • PA is highly effective in stabilizing demineralized dentin collagen against enzymatic attack.
  • PA's non-covalent interaction with collagen likely contributes to its stabilizing properties.
  • PA shows significant promise as a primer for dental adhesive systems.