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Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Published on: September 26, 2013
A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells.
Forum S Shah1, Xiying Wu, Marilyn Dietrich
1Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Cytotherapy
|June 4, 2013
Summary
A novel washing method effectively isolates adipose-derived stem cells (ASCs) without collagenase, offering a comparable cell product and potentially favorable immunophenotype, despite lower cell yield.
Area of Science:
- Cell Biology
- Biotechnology
Background:
- Current isolation of human adipose-derived stem cells (ASCs) uses collagenase, an expensive and time-consuming enzyme.
- Collagenase digests collagen in adipose tissue, impacting cell isolation.
Purpose of the Study:
- To evaluate an alternative method for ASC extraction without enzymatic digestion.
- To compare the washing method with collagenase digestion regarding processing time, yield, differentiation, and immunophenotype.
Main Methods:
- Adipose tissue was washed to isolate and characterize ASCs.
- The washing method was compared to enzymatic digestion using fluorescence-activated cell sorting and differentiation assays.
Main Results:
- The washing method yielded ASCs with a distinct and potentially favorable immunophenotype compared to collagenase digestion.
- Passaged ASCs from both methods showed comparable immunophenotypes and differentiation potential (adipogenic and osteogenic).
Conclusions:
- While collagenase increases cell yield, the washing method produces a similar quality ASC product.
- The non-enzymatic washing method offers a viable alternative for ASC isolation.
