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Related Experiment Video

Updated: May 10, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
12:08

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Published on: March 18, 2012

Iron release from ferritin by flavin nucleotides.

Galina Melman1, Fadi Bou-Abdallah, Eleanor Vane

  • 1Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA. amelman@clarkson.edu

Biochimica Et Biophysica Acta
|June 4, 2013
PubMed
Summary

Iron release from ferritin by reduced flavin nucleotide (FMNH2) is slow with oxygen but rapid without it. FMNH2 may not be a major in-vivo iron release factor under normal conditions.

Keywords:
FMN/NAD(P)HFerritinIron coreIron releaseOxygen consumptionReductive mobilization

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Last Updated: May 10, 2026

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Published on: February 24, 2018

Area of Science:

  • Biochemistry
  • Cell Biology
  • Mineral Metabolism

Background:

  • In vitro studies extensively explore ferritin's iron uptake and core formation.
  • In vivo iron mobilization from ferritin remains poorly understood.
  • Proposed iron-reductive pathways include flavin mononucleotides, ascorbate, and glutathione.

Purpose of the Study:

  • Investigate the kinetics of iron release from ferritin by reduced flavin nucleotide (FMNH2).
  • Discuss the physiological significance of FMNH2-mediated iron release in vivo.

Main Methods:

  • Measured iron release from horse spleen and recombinant human ferritin via UV-Vis spectrophotometry.
  • Monitored oxygen consumption using a Clark oxygen microelectrode.

Main Results:

  • Reductive iron mobilization by the FMN/NAD(P)H system is slow with oxygen, potentially involving superoxide radicals.
  • A rapid phase of iron mobilization by FMNH2 occurs under anaerobic conditions.
  • FMNH2 alone appears insignificant for in vivo ferritin iron release under normoxic conditions.

Conclusions:

  • FMNH2's role in vivo ferritin iron removal is limited by rapid oxidation by molecular oxygen.
  • The primary in vivo ferritin iron mobilization pathway remains unclear.
  • Further research is needed to determine the predominant iron release mechanism under cellular conditions.