Related Experiment Video
Updated: May 10, 2026
08:46
A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
Published on: December 2, 2022
An electrochemiluminescence assay for gp340 (DMBT1)
Cheryl Barber1, Drew Weissman, Kurt Barnhart
1Department of Basic Sciences, College of Dentistry, New York University, New York, NY 10010, USA.
Analytical Biochemistry
|June 4, 2013
Summary
This study developed a sensitive assay for gp340, an innate immune molecule and tumor suppressor. The assay found no correlation between gp340 levels in saliva and vaginal lavage fluid from healthy individuals.
Area of Science:
- Immunology
- Biochemistry
- Oncology
Background:
- Gp340 is an innate immune molecule and a tumor suppressor.
- Scavenger receptor cysteine-rich (SRCR) family proteins play roles in immunity and cancer.
Purpose of the Study:
- To develop a sensitive assay for quantifying gp340 levels.
- To investigate the correlation between gp340 concentrations in saliva and cervical/vaginal lavage fluid.
Main Methods:
- Development of a picogram-level electrochemiluminescence assay using the MesoScale Discovery platform.
- Antibody selection and optimization for the assay.
- Analysis of whole-mouth stimulated saliva and cervical/vaginal lavage samples from healthy volunteers.
Main Results:
- A sensitive and reliable assay for gp340 was successfully developed.
- Analysis of paired saliva and vaginal lavage samples revealed no significant correlation in gp340 concentrations.
- Gp340 levels in saliva do not appear to reflect levels in the female reproductive tract.
Conclusions:
- The developed assay enables sensitive measurement of gp340.
- There is no direct correlation between gp340 concentrations in oral and vaginal fluids.
- Further research is needed to understand gp340 distribution and function in different bodily fluids.
